Antioxidant and xenobiotic proteins driven hydrocarbon degradation in petroleum contaminated soil by bio-film forming Bacillus cereus BY-6

Human activities introduce petroleum hydrocarbons into the environment, where they are recognized as hazardous compounds. Bacteria can degrade hydrocarbons by utilizing carbon and nitrogen off which these hydrocarbons are made off. Based on above facts, aim of this study was to observe the mechanism used by Bacillus cereus strain BY-6 for hydrocarbon degradation in petroleum contaminated soil. Present study reported degradation of compounds such as dodecane, nonane, n-hexadecanoic acid and naphthalene by Bacillus cereus strain BY-6 after 20 days of incubation in petroleum contaminated soil. Formation of vinyl carbon, aldehyde protons, boron nitrite and dibarium calcium tetranitridotungstate along with bio-film formation through expression of chemotaxis sensory proteins were mechanisms used by Bacillus cereus strain BY-6 for hydrocarbon degradation in petroleum contaminated soil. Degradation, xenobiotic and antioxidant proteins and genes such as superoxide dismutase, catalase, xenobiotic reductase, alcohol dehydrogenase, alkane monoxygenase, efflux pump etc were identified through liquid chromatograph—tendem mass spectrometry (nano-LC-MS/MS) and polymerase chain reaction (PCR). Based on above results, it is concluded that strain BY-6 can be utilized for mass production which may restore petroleum contaminated soil to its natural form.