TY - JOUR
T1 - Detection of genetic divergence among putative ethyl methane sulfonate mutants of super basmati using microsatellite markers
AU - Hameed, Kiran
AU - Habib, Madiha
AU - Awan, Faisal Saeed
AU - Sadia, Bushra
AU - Hussain, Shabbir
AU - Qamar, Zia ul
AU - Alzahrani, Hind A.S.
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2023/11
Y1 - 2023/11
N2 - Background: Seeds of super basmati were mutagenized with different ethyl methane sulphonate (EMS) doses for creating genetic variability. Methods and results: A total of 48 randomly selected putative EMS mutants of super basmati were analyzed to dissect the genetic diversity by using 25 SSR primers located on twelve chromosomes of rice. SSRs analysis revealed that wide-range of genetic diversity is present among mutants of super basmati. A sum of 91 alleles were identified, out of these, 82 alleles were polymorphic and the rest of nine alleles were monomorphic in nature. The range of allele number was 2–10 with mean of 3.64 alleles/locus. The value of polymorphic information content was range between 0.039 (RM5) and 0.878 (RM44) with mean of 0.439 for each locus. A number of polymorphic markers showed unique bands of various sizes ranges from 75 to 1000 bp, during genetic dissection of mutant population. Dendrogram divided whole mutant population into four major groups. Phylogenic analyses revealed that 40–96%genetic similarity is present among individuals of mutant population. Conclusion: It is concluded that EMS induced genetic variability and SSRs markers (RM44, RM154, RM1, RM252, RM334, RM487, RM110 and RM257) could be handy for the selection of rice mutants as parents for functional genomic and molecular breeding program.
AB - Background: Seeds of super basmati were mutagenized with different ethyl methane sulphonate (EMS) doses for creating genetic variability. Methods and results: A total of 48 randomly selected putative EMS mutants of super basmati were analyzed to dissect the genetic diversity by using 25 SSR primers located on twelve chromosomes of rice. SSRs analysis revealed that wide-range of genetic diversity is present among mutants of super basmati. A sum of 91 alleles were identified, out of these, 82 alleles were polymorphic and the rest of nine alleles were monomorphic in nature. The range of allele number was 2–10 with mean of 3.64 alleles/locus. The value of polymorphic information content was range between 0.039 (RM5) and 0.878 (RM44) with mean of 0.439 for each locus. A number of polymorphic markers showed unique bands of various sizes ranges from 75 to 1000 bp, during genetic dissection of mutant population. Dendrogram divided whole mutant population into four major groups. Phylogenic analyses revealed that 40–96%genetic similarity is present among individuals of mutant population. Conclusion: It is concluded that EMS induced genetic variability and SSRs markers (RM44, RM154, RM1, RM252, RM334, RM487, RM110 and RM257) could be handy for the selection of rice mutants as parents for functional genomic and molecular breeding program.
KW - Ethyl methane sulphonate mutagenesis
KW - Genetic diversity
KW - Microsatellite markers
KW - Polymorphism
KW - Super basmati
UR - https://www.scopus.com/pages/publications/85169318221
U2 - 10.1007/s11033-023-08425-1
DO - 10.1007/s11033-023-08425-1
M3 - Article
C2 - 37658932
AN - SCOPUS:85169318221
SN - 0301-4851
VL - 50
SP - 8799
EP - 8808
JO - Molecular Biology Reports
JF - Molecular Biology Reports
IS - 11
ER -