Direct Comparison of Four Hematopoietic Differentiation Methods from Human Induced Pluripotent Stem Cells

Melinda L. Tursky; To Ha Loi; Crisbel M. Artuz; Suad Alateeq; Ernst J. Wolvetang; Helen Tao; David D. Ma; Timothy J. Molloy

doi:10.1016/j.stemcr.2020.07.009

Direct Comparison of Four Hematopoietic Differentiation Methods from Human Induced Pluripotent Stem Cells

Melinda L. Tursky
, To Ha Loi
, Crisbel M. Artuz
, Suad Alateeq
, Ernst J. Wolvetang
, Helen Tao
, David D. Ma^*
, Timothy J. Molloy^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Tursky and colleagues addressed a vital, unmet need to aid method selection for iPSC hematopoietic differentiation for research and potentially clinical use, comparing four representative serum- and feeder-layer-free methods. Assessments include production efficiency of CD34⁺ and functional hematopoietic progenitor cells, cost-benefit analysis, and ability to recapitulate aberrant hematopoiesis. An optimized method proved the most efficient, cost-effective, and sensitive.

Original language	English
Pages (from-to)	735-748
Number of pages	14
Journal	Stem Cell Reports
Volume	15
Issue number	3
DOIs	https://doi.org/10.1016/j.stemcr.2020.07.009
State	Published - 8 Sep 2020
Externally published	Yes

Keywords

Down syndrome
cell differentiation
disease modeling
erythroid progenitor cells
hematopoiesis
hematopoietic progenitor cells
hematopoietic stem cells
induced pluripotent stem cells
pluripotent stem cells
β-thalassemia

Access to Document

10.1016/j.stemcr.2020.07.009

Cite this

@article{d3844476c4094f6ca3f9b015e05ddf50,

title = "Direct Comparison of Four Hematopoietic Differentiation Methods from Human Induced Pluripotent Stem Cells",

abstract = "Tursky and colleagues addressed a vital, unmet need to aid method selection for iPSC hematopoietic differentiation for research and potentially clinical use, comparing four representative serum- and feeder-layer-free methods. Assessments include production efficiency of CD34+ and functional hematopoietic progenitor cells, cost-benefit analysis, and ability to recapitulate aberrant hematopoiesis. An optimized method proved the most efficient, cost-effective, and sensitive.",

keywords = "Down syndrome, cell differentiation, disease modeling, erythroid progenitor cells, hematopoiesis, hematopoietic progenitor cells, hematopoietic stem cells, induced pluripotent stem cells, pluripotent stem cells, β-thalassemia",

author = "Tursky, \{Melinda L.\} and Loi, \{To Ha\} and Artuz, \{Crisbel M.\} and Suad Alateeq and Wolvetang, \{Ernst J.\} and Helen Tao and Ma, \{David D.\} and Molloy, \{Timothy J.\}",

note = "Publisher Copyright: {\textcopyright} 2020 The Authors",

year = "2020",

month = sep,

day = "8",

doi = "10.1016/j.stemcr.2020.07.009",

language = "English",

volume = "15",

pages = "735--748",

journal = "Stem Cell Reports",

issn = "2213-6711",

number = "3",

}

TY - JOUR

T1 - Direct Comparison of Four Hematopoietic Differentiation Methods from Human Induced Pluripotent Stem Cells

AU - Tursky, Melinda L.

AU - Loi, To Ha

AU - Artuz, Crisbel M.

AU - Alateeq, Suad

AU - Wolvetang, Ernst J.

AU - Tao, Helen

AU - Ma, David D.

AU - Molloy, Timothy J.

PY - 2020/9/8

Y1 - 2020/9/8

N2 - Tursky and colleagues addressed a vital, unmet need to aid method selection for iPSC hematopoietic differentiation for research and potentially clinical use, comparing four representative serum- and feeder-layer-free methods. Assessments include production efficiency of CD34+ and functional hematopoietic progenitor cells, cost-benefit analysis, and ability to recapitulate aberrant hematopoiesis. An optimized method proved the most efficient, cost-effective, and sensitive.

AB - Tursky and colleagues addressed a vital, unmet need to aid method selection for iPSC hematopoietic differentiation for research and potentially clinical use, comparing four representative serum- and feeder-layer-free methods. Assessments include production efficiency of CD34+ and functional hematopoietic progenitor cells, cost-benefit analysis, and ability to recapitulate aberrant hematopoiesis. An optimized method proved the most efficient, cost-effective, and sensitive.

KW - Down syndrome

KW - cell differentiation

KW - disease modeling

KW - erythroid progenitor cells

KW - hematopoiesis

KW - hematopoietic progenitor cells

KW - hematopoietic stem cells

KW - induced pluripotent stem cells

KW - pluripotent stem cells

KW - β-thalassemia

UR - https://www.scopus.com/pages/publications/85089976938

U2 - 10.1016/j.stemcr.2020.07.009

DO - 10.1016/j.stemcr.2020.07.009

M3 - Article

C2 - 32763163

AN - SCOPUS:85089976938

SN - 2213-6711

VL - 15

SP - 735

EP - 748

JO - Stem Cell Reports

JF - Stem Cell Reports

IS - 3

ER -

Direct Comparison of Four Hematopoietic Differentiation Methods from Human Induced Pluripotent Stem Cells

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this