TY - JOUR
T1 - Exome array identifies functional exonic biomarkers for pediatric dental caries
AU - Borgio, J. Francis
AU - Alsuwat, Hind Saleh
AU - Alamoudi, Widyan
AU - Hegazi, Fatma Mohammed
AU - Al Otaibi, Waad Mohammed
AU - M. Ibrahim, Abdallah
AU - Almandil, Noor B.
AU - Al-Amodi, Amani M.
AU - Alyousef, Yousef M.
AU - AlShwaimi, Emad
AU - Almasoud, Naif
AU - Kamaraj, Balu
AU - Sayed, Abdul Azeez
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2022/2
Y1 - 2022/2
N2 - Background: Pediatric dental caries is common among Arab children, however we are still searching for possible genes and molecular mechanisms that influence caries development. Aim: To identity genetic predispositions of dental caries among Saudi children with high DMFT (Decayed, Missing, and Filled Teeth). Design: This case-control study analysed putative functional exonic-variants (n = 243,345) to study the molecular genetics of pediatric caries with high dmft index, 8.75 ± 4.16 on Arab-ancestry subjects with primary dentition (n = 111; 76 cases, dmft>5 and 35 controls, dmft = 0). Results: Pediatric caries is significantly associated with single nucleotide polymorphisms (SNP) in the GRIN2B-rs4764039C (p-value = 2.03 × 10−08) and CFH-rs1065489G (p-value = 8.26 × 10−08) genes, even after Bonferroni correction. Irregular tooth brushing habits (p = 0.0404) and irregular dental visits (p = 0.0050) are significantly associated with caries. Functional enrichment analysis of significant genes is associated with calcium-activated chloride channel, Staphylococcus aureus infection, and N-linked glycosylation. Conclusion: Genetic predispositions are found to be significantly associated with the high prevalence of pediatric caries, which is a disorder of multigene-environment interaction. The significant functional exonic variants identified can be biomarkers for the early diagnosis of pediatric dental caries in Arabs.
AB - Background: Pediatric dental caries is common among Arab children, however we are still searching for possible genes and molecular mechanisms that influence caries development. Aim: To identity genetic predispositions of dental caries among Saudi children with high DMFT (Decayed, Missing, and Filled Teeth). Design: This case-control study analysed putative functional exonic-variants (n = 243,345) to study the molecular genetics of pediatric caries with high dmft index, 8.75 ± 4.16 on Arab-ancestry subjects with primary dentition (n = 111; 76 cases, dmft>5 and 35 controls, dmft = 0). Results: Pediatric caries is significantly associated with single nucleotide polymorphisms (SNP) in the GRIN2B-rs4764039C (p-value = 2.03 × 10−08) and CFH-rs1065489G (p-value = 8.26 × 10−08) genes, even after Bonferroni correction. Irregular tooth brushing habits (p = 0.0404) and irregular dental visits (p = 0.0050) are significantly associated with caries. Functional enrichment analysis of significant genes is associated with calcium-activated chloride channel, Staphylococcus aureus infection, and N-linked glycosylation. Conclusion: Genetic predispositions are found to be significantly associated with the high prevalence of pediatric caries, which is a disorder of multigene-environment interaction. The significant functional exonic variants identified can be biomarkers for the early diagnosis of pediatric dental caries in Arabs.
KW - Biomarker
KW - Dental caries
KW - Genotype
KW - Haplotype
KW - Multigene-environment interaction
KW - SNPs
KW - Tooth brushing habits
UR - https://www.scopus.com/pages/publications/85118866520
U2 - 10.1016/j.compbiomed.2021.105019
DO - 10.1016/j.compbiomed.2021.105019
M3 - Article
C2 - 34749984
AN - SCOPUS:85118866520
SN - 0010-4825
VL - 141
JO - Computers in Biology and Medicine
JF - Computers in Biology and Medicine
M1 - 105019
ER -